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63285_TTAGGC_S40_L005_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63285_TTAGGC_S40_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63285_TTAGGC_S40_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119112
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT348730.2877521059298734TruSeq Adapter, Index 3 (100% over 52bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51100.038.93368542
TATGCCG51200.038.58810845
CGTATGC51650.038.2514443
CTCGTAT54000.036.71502341
ACACGTC57450.036.30848712
TCTCGTA57300.034.7208240
ATGCCGT57250.034.5900246
ATCGGAA56900.034.131541
CGTCTGA60350.033.9544515
ATCTCGT59000.033.68210239
ACGTCTG60900.033.647814
CACGTCT62800.032.9960113
CACACGT63400.032.9372611
TCGGAAG59850.032.5502932
CTTAGGC62650.032.34509732
ACTTAGG63000.031.94567931
CATCTCG62250.031.81380338
GTATGCC63150.031.57685544
GCACACG68700.030.76448410
CACTTAG66250.030.30947130
Produced by FastQC (version 0.11.5)