63286_GATCAG_S17_L008_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63286_GATCAG_S17_L008_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63286_GATCAG_S17_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340855
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT1606.335066E-512.9465251
CGCGGAT5350.011.1853691
CTCGGTT10900.010.7689751
CCGGGAT15750.010.5216211
GTCGATT5055.456968E-1210.0267921
CGGCAAT7050.09.7940621
CGAGAAT11600.09.3254661
CGGGGAT13600.09.30796051
CGGGAAC16200.09.2348331
CTCGGGT16600.09.0123071
CGGAAAT9200.09.0062791
CGACCTA2300.00183137778.9966974
CTCGAAT12500.08.8381611
CGCAACT8650.08.7806871
ATGCGCG2901.7382552E-48.7209386
CTGGAAT60200.08.6023441
CGAAGAT18400.08.505931
CGACAGT9250.08.4599521
CTCGTTT12300.08.4205051
CTCGGAT15800.08.3032581