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63287_ACTGAT_S18_L008_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63287_ACTGAT_S18_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63287_ACTGAT_S18_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12365434
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG19100.017.10310445
CGTATGC19850.016.57097446
CTCGTAT21650.015.2027244
TATCTCG23000.014.415709541
TCTCGTA24150.014.10844443
CGCGATT1851.7813736E-512.4406551
CGAATCG3202.6375346E-1012.2144223
ATCTCGT28650.012.13474942
GATCGGA24600.012.06895351
CGGGAAT13000.010.6224071
CTCGGAT18200.010.2430331
CTCGGTT11600.010.1187571
ACACGTC34250.010.00225513
CGTCTGA34600.09.90107716
CTCGCAT11200.09.8636631
ACGTCTG35050.09.77395815
GTATGCC26700.09.64893246
GATATAT37050.09.57025137
ATATATC37050.09.38514738
TGATATA40550.09.36709236
Produced by FastQC (version 0.11.5)