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63287_ACTGAT_S42_L005_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63287_ACTGAT_S42_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63287_ACTGAT_S42_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12092631
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15050.019.71420145
CGTATGC15250.019.0036746
CTCGTAT18300.015.96154744
TATCTCG18850.015.74004941
TCTCGTA20750.014.52041943
GATCGGA19850.013.4474761
ATCTCGT24650.012.22312142
CTCGGAT18350.010.1576281
CTCGCAT11350.010.1371871
CGGCAAT9500.09.9312421
CGGGGAT13950.09.7324271
ATATATC31550.09.6962438
GTCCGAT5950.09.6686611
ATCGGAA28350.09.5731912
ACACGTC31350.09.39008813
CCGGAAT12900.09.2759191
GATATAT33300.09.25575237
CGTCTGA31700.09.21393816
CGGAAAT12400.09.0932211
CGCGAAT3307.698494E-69.0651031
Produced by FastQC (version 0.11.5)