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180905_SNK268_A_L006_AOES-9-1_R1.fastq.gz FastQC Report
FastQCFastQC Report
Wed 3 Oct 2018
180905_SNK268_A_L006_AOES-9-1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename180905_SNK268_A_L006_AOES-9-1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48329936
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC24023224.970670766044466No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT22768904.7111380408200825No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA19158423.964089668978664No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC11989952.4808536886951393No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA10548852.1826741090656525No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC9274731.9190445441516828No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC9235291.910883970547778No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA9091101.8810494596971947No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG7492861.5503558705312583No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA6662221.3784872382202202No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6586141.3627454420796252No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC5645441.1681041746051557No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG5259951.0883420164264235No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC5176511.0710773546234367No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA4228730.8749711565932966No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA4056660.839367964402022No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC3980700.8236509975928791No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3376050.6985422037389001No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT3176290.6572096433150667No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA2805190.5804249357996253No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA2686690.5559059709907334No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCAC2527080.5228808910485625No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA2345980.48540929166552177No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA2134520.44165587142511425No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT2103840.4353078390172087No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACCA1919830.3972341283464559No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1893220.3917282240969655No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1855650.38395457424152185No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC1800650.3725744639926691No Hit
GTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1752800.36267376807616714No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC1707410.3532820734544321No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1400760.2898327860396918No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCAC1331420.27548557068231994No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT1222650.2529798508319978No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT1169570.24199700988637765No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA1084710.22443853432787497No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1082110.22390056547974738No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCA1056170.21853329166419752No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1018040.21064377159531103No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA962290.19910847802488296No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT902350.18670622696458775No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA900280.18627792099704002No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA848560.1755764791412097No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCTGTAGGCACC836480.17307699310837077No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA824840.17066854795752265No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA788050.1630562887565173No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC784750.16237348214158612No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA761200.15750072584412278No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCACC668600.13834075840696333No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATAG640650.13255759328959177No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA611370.1264992364152934No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAG610570.12633370753894646No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT607890.12577918580318417No Hit
TGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAG577670.11952633249917816No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA547600.11330451585948717No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA530360.10973736857421039No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCTGTAGGCA523360.10828899090617458No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT520150.10762480628983245No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCTGTAGGC503490.10417766743990722No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT485320.10041809283587713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGC3201950.042.9355161
TTGGGCG184600.041.326031
CCTCCCG895750.041.1954771
TCGTGGG1006950.040.2705761
TGGGCGC3390750.039.577431
CGTGGGG7741550.038.213631
GGACGAG3638950.037.36919819
CGAGGCG3636800.037.2926222
TGGACGA3651000.037.28621718
ACGAGGC3637100.037.2859221
GACGAGG3644700.037.27849220
CGCGCGC3642500.037.2537275
CGCGGCT3663900.037.2263912
GAGGCGC3658650.037.1396723
GGCGCCG3651300.037.09461225
CGGCTGG3692350.037.08014314
GCGCGCG3684250.036.8291664
GGCTGGA3696100.036.8205515
CCGCCGC3653650.036.76764729
GCTGGAC3698450.036.70021416
Produced by FastQC (version 0.11.5)