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180905_SNK268_A_L006_AOES-9-2_R1.fastq.gz FastQC Report
FastQCFastQC Report
Wed 3 Oct 2018
180905_SNK268_A_L006_AOES-9-2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename180905_SNK268_A_L006_AOES-9-2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37835349
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC24407316.450927676126366No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT15528994.1043601844402176No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA12061693.1879420485853056No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC10488132.7720452638087205No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA9526472.5178755454323944No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC9410552.487237530173172No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC7901392.0883618649850435No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA7496921.981459190451765No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC4800481.2687817416458878No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC4786441.2650709261331248No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC3442700.9099162796146006No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3300440.8723165207224598No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA3267900.8637160978745037No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA3067870.8108475489415996No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2665020.7043730454290246No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2561990.6771418971184857No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT2345630.6199572785756515No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA2295310.6066575466239257No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCAC2188700.5784801932182521No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2119230.560119056916853No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1850750.48915896084373367No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT1803950.47678957580119063No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA1597800.4223034919011848No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT1596050.42184096147758543No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1572740.41568005623524185No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC1564370.41346783929494085No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1493040.39461509922903054No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC1475620.39001093924097274No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACCA1361690.35989888714915774No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1331350.3518799311194407No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT1268930.3353821316673992No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCAC1265180.33439099504540054No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1229810.3250425944267093No Hit
GTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1114510.29456844708899077No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA1051980.2780415742960373No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCA913970.241565103575495No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA838650.2216577941437781No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA825050.21806327199466297No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC641500.16955043813656906No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT599200.1583704170404243No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA593960.15698546880061817No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCTGTAGGCACC582870.15405434743049415No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA550340.14545656761352987No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA525790.13896792652817871No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA518820.13712573392675723No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCACC514710.1360394481890467No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT500750.13234977692421973No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCTGTAGGC496730.13128727846543717No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA486870.12868124990732874No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT477980.12633159535544392No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC438250.11583083322424222No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATCA430390.11375341086453306No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCTGTAGGCA404590.10693439090518235No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAA393890.1041063477437462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGC3258600.043.1256681
TTGGGCG148000.041.6585661
TCGTGGG827800.040.516461
TGGGCGC2638150.039.6919821
CCTCCCG336300.038.646491
ATAAACG99700.038.209121
CGTGGGG5648200.037.476441
GGACGAG2831150.037.4750319
CGAGGCG2825950.037.45722622
TGGACGA2836750.037.44833818
CGCGCGC2828500.037.4201475
GACGAGG2834150.037.40927520
ACGAGGC2830900.037.3979421
CGCGGCT2851500.037.38574212
GAGGCGC2844150.037.29195823
GGCGCCG2844050.037.21080425
CGGCTGG2876200.037.1709814
GCGCGCG2846250.037.1651734
GGCTGGA2874850.036.9527915
GCTGGAC2877400.036.83366416
Produced by FastQC (version 0.11.5)