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180905_SNK268_A_L006_AOES-9-3_R1.fastq.gz FastQC Report
FastQCFastQC Report
Wed 3 Oct 2018
180905_SNK268_A_L006_AOES-9-3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename180905_SNK268_A_L006_AOES-9-3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43460625
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATC21158304.868383738153788No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAA20711924.765674676790773No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC12087812.7813244747400665No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATC8970802.0641212591857574No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAA7014841.6140679062944907No Hit
AGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACCTGTAGGCACCATC5309421.2216621367042007No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCCTGTAGGC5124221.179048851690467No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAAT5045981.1610463494254857No Hit
ACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCCTGTAGGCACCA5043141.16039288436372No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCA4545631.0459191509556063No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAG4139760.9525311704560162No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAAT3895640.8963607863439608No Hit
AAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCCTGTAGGCACCAT3812140.8771479931547234No Hit
AGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTCTGTA3739520.8604386154133771No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT3654050.8407725383608726No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA3419000.7866891007664985No Hit
AAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCAC3140670.7226472237801459No Hit
AGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCCTGTAGGCACCATCAA3018190.6944653925248429No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA2994690.6890581992320635No Hit
AACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCAC2959960.6810670578253304No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC2773020.6380534104145994No Hit
ACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCCTGTAGGCA2555560.5880173145232035No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAA2504920.5763653882105929No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2475280.5695454218617427No Hit
CGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCCTGTAGGCACCATCAAT2353870.5416097904712599No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATCTGTAGGCACC2274560.5233610883414586No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA2266910.5216008743546602No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA2196040.5052941599436271No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAATA2182520.5021832981002919No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA2109760.48544170729252056No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCCTGTAGGCACCAT2015750.46381063318808696No Hit
ACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCTGTAGGCACC1911190.43975207443519276No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1721960.39621151329508036No Hit
ACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCTGTAGGCACCAT1668150.38383019112127353No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1606830.36972086802709353No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA1581540.36390180767073643No Hit
ACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCCTGTAGGCACCA1549030.3564214734601723No Hit
AACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCCTGTAGGC1461750.336338927477458No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCCTGTAGGCA1402170.3226299667802752No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCTGT1392600.3204279735967902No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCCTGTAGGCACC1382020.31799358614838147No Hit
CCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCCTGTAGGCACCATCAA1351630.3110010498008255No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1305820.30046047427987976No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT1299010.29889353869163177No Hit
AAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCTGTAGGCACCATCAA1140400.2623984353653451No Hit
GAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATCA1107230.25476623955591987No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCACC1072060.24667385708327944No Hit
CCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCCTGTAGGCACCAT1068770.2459168500222903No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1048360.24122064512417848No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCCTGTAGGCAC1023770.2355626500999468No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA925100.21285934107022159No Hit
GATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTCTGTAGGCACCATC902840.2077374635086357No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC853700.19643067719343657No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC843720.1941343457439924No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC822810.18932309418007678No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT822500.18925176524727844No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTCTGTAGGCACCATCAAT805770.18540230380948272No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTCTGTAGGCACCATCAA796620.1832969498252729No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCCTGTAGGCACCA792740.18240418769863526No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCCTGTAGGCACCAT787710.18124681824064887No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG783600.1803011346477415No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCAC778120.17904022319053164No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT772200.17767807066741448No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCTGTAGGCACCATCA757310.17425198095977681No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCCTGTAGGCAC753550.1733868300329321No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA724740.1667578411493162No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCCTGTAGGCACCA722400.16621942275335433No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATCTGTA713230.16410946690251232No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACCA712030.16383335490458317No Hit
ATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCCTGTAGGCACCATC692420.1593212246717575No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTCTGTAGGCACCA643510.14806735982282815No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCCTGTAGGCACCATCAAT637540.14669370263313056No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA618270.14225980413305148No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCCTGTAGGCACCATCAATAGA617930.14218157240030488No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCCTGTAGGC615560.14163625120439477No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATCTGTAGGCACCATC603740.13891654802479256No Hit
ATTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCCTGTAGGCACCATCAA581440.13378546672994232No Hit
GCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACCTGTAGGCACCATCA563590.12967830076074607No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCCTGTAGGCACCATCAATAG548030.1260980485209313No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA539820.12420898226843263No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCTGTAGGCACCAT523960.12055970202913557No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCTGTAGGC506290.11649395285962869No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTCTGTAGGCACCATCAA503920.1159486316637186No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG494120.11369371701396377No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCTGTAGGCACCATCAAT493450.11353955448178668No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA489660.11266750075499374No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCCTGTAGGCACCATCA477830.10994549664207544No Hit
AGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCCTGTAGGCACCATCAA466220.10727411306211082No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC452460.10410802881918979No Hit
AGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTCTGTAGGCAC450410.10363633748939415No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT443050.10194285056876196No Hit
CGCCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCCTGTAGGCACCATC440720.10140673310611618No Hit
AGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCCTGTAGGCACCATC436570.10045184577994448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACGA645250.043.6736831
ATGTACA242350.043.3928071
ATAAACG4197100.042.770121
ACGTGCG734850.042.4777641
TATAAAC86350.042.0553321
CCAGTGC1928900.041.805081
AGCGCCG1275500.041.6176151
AGAGCGA209150.041.5253451
AAAACGG47050.041.4590341
TAAACGA4353900.041.2196122
AGAATAA200800.040.25631
CGTGGAA463850.040.21833432
TGGCGTG573500.040.07895717
CTGGCGT575800.039.91108716
GCAGAAC396350.039.8789633
GGGCGTG478700.039.48642729
GCGCCGA1253400.039.440632
GGTTTTA182450.039.27080521
CGTGCGC795100.039.2111972
GTGGAAT471350.039.1833
Produced by FastQC (version 0.11.5)