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180905_SNK268_A_L006_AOES-9-4_R1.fastq.gz FastQC Report
FastQCFastQC Report
Wed 3 Oct 2018
180905_SNK268_A_L006_AOES-9-4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename180905_SNK268_A_L006_AOES-9-4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43150
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC23965.552723059096176No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT13533.1355735805330243No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11902.757821552723059Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA10792.500579374275782No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA9682.2433371958285053No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC9492.1993047508690617No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC8411.9490150637311705No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA6431.4901506373117035No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC6281.455388180764774No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC4441.0289687137891077No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC4280.9918887601390498No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3740.8667439165701042No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3350.7763615295480881No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC3280.7601390498261877No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3050.7068366164542295No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2940.6813441483198146No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA2730.6326767091541136No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2620.6071842410196987No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2560.593279258400927No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCAC2100.48667439165701043No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA2060.47740440324449596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT2010.46581691772885286No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1790.4148319814600232No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT1600.3707995365005794No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACCA1550.35921205098493625No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT1550.35921205098493625No Hit
CCAGTGCGCCCCGGACGGGTCGCGCCGTCGGGCCCGGGGGCAGTAGGCAC1520.3522595596755504No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA1430.3314020857473928No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC1340.3105446118192352No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1330.3082271147161066No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC1300.3012746234067208No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT1290.2989571263035921No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1260.29200463499420626No Hit
GTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1230.2850521436848204No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCAC1180.27346465816917725No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1100.2549246813441483No Hit
CCAGTACGCCCCGGACGGGTCGCGCCGTCGGGCCCGGGGGCAGTAGGCAC870.20162224797219006No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA840.19466975666280417No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACCA830.19235225955967555No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA800.1853997682502897No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG790.18308227114716108No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA760.1761297798377752No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA710.1645422943221321No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA690.15990730011587487No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA640.14831981460023175No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC640.14831981460023175No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG560.12977983777520277No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA560.12977983777520277No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCTGTAGGC530.12282734646581692No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCACC530.12282734646581692No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA530.12282734646581692No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCTGTAGGCACC520.1205098493626883No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATCA500.11587485515643105No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT490.11355735805330244No Hit
CCAGTGCGCCCCGGACGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC480.11123986095017381No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCTGTAGGCA450.10428736964078794No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATC450.10428736964078794No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA440.10196987253765932No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT440.10196987253765932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAC351.3783574E-744.0998841
CCTCCCG551.8189894E-1244.0998841
TGTGGGG207.685929E-444.0998841
CTCGCCG207.685929E-444.0998841
AGCCCCG207.7744597E-443.9976843
ACGCACC207.7744597E-443.99768441
CGCTCTA207.7744597E-443.99768436
ACACCCC800.043.99768415
GACACCC800.043.99768414
TACGCAC207.7744597E-443.99768440
GGAGGGC454.5656634E-1043.9976844
TGGGAGG408.001734E-943.9976842
GGCAGTA408.001734E-943.99768439
AATCCTT254.373154E-543.9976814
CCGGACG600.043.9976811
ACGCCCC351.4038233E-743.997686
GCCCAAA254.373154E-543.997689
CCCGGAC600.043.9976810
CAGTACG351.4038233E-743.997682
GACGGGT600.043.9976814
Produced by FastQC (version 0.11.5)