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26682_ID1144_2_S50_L006-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26682_ID1144_2_S50_L006-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26682_ID1144_2_S50_L006-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597771
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC65490.11699299596214278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC39950.032.326621
ATTACGG21300.025.9949840-41
CGGAATA22000.024.3255144
GCGGAAT22900.023.1175443
TCCGATC37000.023.0480277
CCCCCGT16950.022.994781
CCGATCT38750.021.4058258
TTACGGC21100.021.0201142-43
TCTTCCG63250.020.2674628
TTCCGAT42300.020.1721216
CGTAACG6150.019.79747840-41
CGGGGGA38800.019.341141
GTCGGAA30250.018.6919126-27
CTTCCGA69200.018.1891969
CGATCTA6450.017.9861378
GGGGGGT92700.017.7595041
CTATACT31450.017.7439268-69
GTGTGCT73900.017.7119372
GTGCTCT76900.017.397964
GGCAAGT53200.017.3853074
Produced by FastQC (version 0.11.5)