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26683_ID1144_3_S11_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26683_ID1144_3_S11_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26683_ID1144_3_S11_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034558
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN270980.15025596967777088No Hit
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC208580.11565573162369712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC140150.036.241891
ATTACGG66700.025.44871740-41
TCCGATC138450.023.3612867
TCTTCCG216750.022.7422948
CCGATCT147000.021.6470728
CGGAATA79500.021.3696754
GCGGAAT81650.020.8077813
GTGTGCT244000.020.7218672
GTGCTCT245600.020.6574694
TTACGGC67850.020.58588842-43
TTCCGAT160000.020.2591956
CTTCCGA242500.019.9210229
TGTGCTC259800.019.3952313
CGGGGGA113950.017.4533821
CGTAACG21050.017.44637740-41
GGGGGTA171250.017.369471
CCCCCGT50950.017.2981321
GTCGGAA102100.017.16417126-27
GGTTCGA52000.017.0602534
CGATCTA25800.017.04249
Produced by FastQC (version 0.11.5)