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26684_ID1144_4_S12_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26684_ID1144_4_S12_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26684_ID1144_4_S12_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18072289
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG65200.027.28679840-41
TCCGATC100450.025.0181856
CCGATCT108800.022.9818137
TTACGGC66050.022.20637142-43
CGTGTGC112750.020.9908221
TTCCGAT121800.020.5735995
CCCCCGT50450.019.8916471
CGGAATA65950.019.4379844
GTCGGAA97150.018.90926-27
CGATCTA19850.018.6604659
GCGGAAT67150.018.4004763
AATTACG79200.018.18985440-41
CTATACT101800.017.790668-69
GGCAAGT171200.017.7542084
CGTAACG18200.017.64317140-41
GTGTCGG106250.017.56204424-25
TAATTAC106550.017.43003338-39
CGGGGGA116350.017.3499681
GGTTCGA50500.017.1146744
GGGGGGT292200.016.9933641
Produced by FastQC (version 0.11.5)