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26685_ID1144_5_S52_L006-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26685_ID1144_5_S52_L006-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26685_ID1144_5_S52_L006-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524639
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC37680.10690456526186087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG12450.026.62512240-41
TCCGATC18850.025.106686
CCGATCT19850.024.1175297
TTACGGC12800.022.29370142-43
TTCCGAT23150.020.9803315
CGGGGGA23200.020.4375321
CGTAACG4950.020.38102140-41
CGGGGGG34900.020.3789731
CCCCCGT7950.020.3654271
CGTGTGC21800.019.0975911
AATTACG14800.019.08655440-41
GTCGGAA18000.019.06085826-27
GTGTCGG19550.018.13928624-25
CGCGTCG2251.5118143E-417.989461
GGCAAGT32100.017.8325824
TGTCGGA17200.017.4330326-27
GGGGGTA34050.016.9818691
CGATTGC12050.016.6734384-85
CTATACT20350.016.63366768-69
CAAGTGA31300.016.5952956
Produced by FastQC (version 0.11.5)