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26686_ID1144_6_S53_L006_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26686_ID1144_6_S53_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26686_ID1144_6_S53_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9879929
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC65250.033.4021157
TTCCGAT66400.032.8400886
CCGATCT72650.030.0749918
CGATCTA17500.024.7716469
CGTGTGC59550.024.0511
TCTTCCG100450.022.1996214
CGATCTC37500.021.1164539
CTTCCGA109900.020.3434415
CGATCTG39250.017.6714959
CGTTGAT15350.013.5991591
TGCTCTT169750.013.2537831
TAATCGT10450.012.2074331
GCTCTTC201400.011.6186762
CGATCTT25350.011.4004969
GTGTGCT125100.011.38970852
GTGCTCT127600.011.25733954
TCGTTGA12500.011.1207994
TGTGCTC135750.010.7521553
TACGCCC23450.09.16583108-109
ACTAGAT23300.08.9591031
Produced by FastQC (version 0.11.5)