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26687_ID1144_7_S54_L006_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26687_ID1144_7_S54_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26687_ID1144_7_S54_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803698
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47700.12540427762666753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC27850.035.9235231
TCCGATC26600.025.3711036
TTCCGAT27000.025.0298615
TCTTCCG40850.024.4142428
CCGATCT29050.023.4188257
CTTCCGA43600.022.6093149
CGATCTA6800.019.4988128
GTGCTCT51800.019.4024164
GTGTGCT51900.019.2537382
CGTTGAT7400.018.7558861
TGTGCTC55250.018.0863173
CGATCTC14550.017.433278
TGCTCTT66650.015.3394385
CGATCTT10350.013.9247968
GCTCTTC77700.012.9912876
CGTAACG1600.002238843112.61097640-41
CGATCTG15050.012.2575228
TCGCTAA8150.010.805663110-111
GCGGAAT5055.33333E-410.2940793
ATATCGG9800.010.29209928-29
Produced by FastQC (version 0.11.5)