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26688_ID1144_8_S16_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26688_ID1144_8_S16_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26688_ID1144_8_S16_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14631527
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC163710.11188852674092048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC131950.046.177531
TCTTCCG186250.032.150968
CTTCCGA203000.028.501739
GTGTGCT217700.028.3343642
GTGCTCT215100.028.1925284
ATTACGG54200.026.73148740-41
TGTGCTC228150.026.5551243
TCCGATC121500.023.59396410-11
CGGAATA59850.022.5270824
CCGATCT128450.022.36010412-13
TTACGGC54550.022.32826642-43
GCGGAAT61900.021.4075493
TGCTCTT290900.021.0852285
TTCCGAT141000.020.5766610-11
CCCCCGT42300.020.245711
CGATCTA18500.019.9720712-13
AATTACG63650.019.13518740-41
GTCGGAA78250.018.77537226-27
CTATACT81700.018.1491368-69
GTGTCGG83700.017.96696724-25
Produced by FastQC (version 0.11.5)