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4R013-L2-P04-TGACCA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P04-TGACCA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P04-TGACCA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9530673
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA2443592.563921771316674No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG2237702.347892955723064No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA2070812.1727846501500996No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1858721.9502505227070532No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC499900.5245169989569467No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT400220.4199283723195623No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG365010.38298449647784577No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG246470.2586071308920157No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG239910.25172409125777373No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG223800.23482077288770686No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG219210.23000474363143084No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT195640.2052740661651071No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA173140.18166607961473447No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA167440.17568538968864003No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC154640.1622550684510947No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT151740.15921226129571334No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC142950.14998940788336773TruSeq Adapter, Index 4 (100% over 50bp)
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA130130.13653810176888873No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA124490.13062036647359532No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC113800.11940394975255159No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT112810.11836519834433519No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG109170.11454595074240823No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC107920.11323439593405417No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG101620.10662415969994983No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC99010.10388563326010662No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT97070.10185010019754114No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT97070.10185010019754114No Hit
GCTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATC96810.1015772967974035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT234300.076.865931
GGATCTC251350.071.446762
GATCTCT287000.062.604353
GACGCTC99300.061.150172
GCGCCCC328900.058.66699694
ACGCTCA109700.055.9523933
CGACGCT42750.053.768541
GCTCAAA469250.052.004541
ATCTCTT352250.050.9671024
TCTCTTG352800.050.927615
GCATTTC396450.050.44893694
CTCTTGG370950.048.3952526
CGCCGGG7150.045.073941
TCTTGGT462050.038.9949877
CGCATTT622600.038.38138694
CGCTCAA169000.037.5148124
CTCAAAC672300.036.3796542
CAGGCAT671650.036.1317378
ACAGGCA677000.035.832327
AGGCATG678400.035.7653059
Produced by FastQC (version 0.11.5)