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4R013-L2-P05-ACAGTG-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P05-ACAGTG-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P05-ACAGTG-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872157
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG3266673.681934393180824No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA2995503.3762928225909437No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA2860723.224379370202759No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG2357982.657730245305623No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC540930.6096938996909094No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG480100.5411310913456558No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT448770.5058183708877109No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT317250.35757933499147954No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG300750.3389818282070527No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG297170.33494673279564374No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG290320.32722594967604834No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG267120.3010767280155209No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA217370.24500242725641577No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA199840.2252439851999914No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT199290.22462406830717715No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC181570.20465147314232607No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC162770.18346158662431244No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA158630.17879530310385625No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT145040.16347772024322835No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA143420.1616517832134846No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC142280.1603668645629242No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT136100.15340125293093887No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT128690.1450492817022963No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC127910.1441701268361234No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG127050.14320080224008658No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA126830.1429528354829609No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC120340.13563781614775303No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG118740.13383442155047526No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG117430.1323578922239541No Hit
TGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAGACACGGATT115550.13023890357215273No Hit
GCTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATC112960.12731965856780939No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC110880.1249752455913483No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA109940.12391575126544763No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA106450.11998209680013552No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG101840.11478606611672901No Hit
CAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCGCC98570.11110037840854259No Hit
CCCAGAACTACCTTGCCGCACCAGACAGAGAGACGGATTCCTCACGCCTC96360.1086094396210527No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA91780.10344722258634513No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT91710.10336832407271423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT286250.079.2612151
GGATCTC308050.073.387052
GATCTCT348000.064.989323
CGACGCT45050.064.584111
GACGCTC114900.064.098012
GCGCCCC448000.062.70858494
ACGCTCA124800.059.3522533
TCTCTTG398900.056.4717985
ATCTCTT403500.055.921824
GCTCAAA516500.055.0927931
GCATTTC549750.053.66673394
CGCCGGG7650.052.0319331
CTCTTGG435000.051.8670466
CTGCATG23350.044.2771848
TCTTGGT522250.043.282357
CGCTCAA182200.041.5050354
CGTGCGT12250.039.4562841
GTTCTCG812400.039.3335725
GGTTCTC819450.039.0931174
TCTCGCA822150.038.9889147
Produced by FastQC (version 0.11.5)