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4R013-L2-P14-AGTTCC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P14-AGTTCC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P14-AGTTCC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11446263
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG1978711.7286952082090024No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA1725911.5078370993222854No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA1568191.3700454025912212No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1479561.292614017343477No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG377110.3294612398823965No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC285500.24942638483844026No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG284600.24864010201408093No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT255810.22348778811040773No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA231840.2025464555549702No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG206700.18058295532786553No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC199170.17400438903072557No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC172240.15047705963072838No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG158780.1387177631686429No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT157660.13773927787610682No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT157340.13745971064966792No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG141620.12372597065085783No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG140590.12282611364075768No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC138870.1213234397986487No Hit
TGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAGACACGGATT133640.11675426294153822No Hit
CAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCGCC132690.11592429773804777No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG125780.10988739294213315No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG122810.10729265962174728No Hit
GCTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATC117770.10288947580533489No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT117540.10268853686133195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT200700.074.081191
GGATCTC213600.069.244262
GCGCCCC275700.061.24342394
GATCTCT250050.059.2441943
GACGCTC80750.049.239792
GCATTTC359950.048.85405794
TCTCTTG313150.047.0346375
CTCTTGG315200.046.561476
GCTCAAA334800.046.4191061
ATCTCTT319700.046.277414
CGACGCT35750.045.7387051
ACGCTCA92650.043.2703133
CGCCGGG9850.042.3435481
TCTTGGT399900.037.5455477
GTTCTCG503900.037.437285
TCGCATC506300.037.257059
CTCGCAT514500.036.6541188
TCTCGCA528550.035.7597967
CTCAAAC516000.035.1519621
CAGGCAT504850.035.1207247
Produced by FastQC (version 0.11.5)