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4R013-L2-P15-ATGTCA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P15-ATGTCA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P15-ATGTCA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18996746
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG5683362.9917544825834907No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA4975762.619269637020993No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA4899012.57886798086367No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG4314312.2710784257472305No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC862310.45392510906867944No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG850090.44749242844011283No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT826700.43517979342356844No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT666300.3507442800993391No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG553990.2916236285940761No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG528880.2784055753548529No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG466950.24580525527898306No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG398950.2100096511265666No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC383680.20197143237057547No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT362580.19086426696445802No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA327860.17258745260898894No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT323980.1705449975485275TruSeq Adapter, Index 15 (97% over 40bp)
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA315250.16594947366248936No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC291760.15358419805160314No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT258800.13623385815654954No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT254260.13384397517343233No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA253670.1335333956668158No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC250900.13207525120354824No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA242070.12742708672316827No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA237310.12492139443249911No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA233490.1229105237286428No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC233080.12269469729184145No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT230540.12135762619556002No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA220560.11610409488024948No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG201120.10587076334020575No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC194280.10227014668722739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT534100.079.667251
GGATCTC574450.073.896792
GATCTCT648900.065.4470443
GACGCTC185000.061.0232242
GCGCCCC805400.058.47086794
CGACGCT80900.057.2486951
TCTCTTG750200.056.345675
ATCTCTT751700.056.3085564
ACGCTCA204400.055.4610333
GCTCAAA870500.055.00121
CTCTTGG797600.052.9814536
GCATTTC987150.048.19103294
TCTTGGT978750.043.5151337
CGCCGGG14750.042.1016241
CGCTCAA295400.039.1232574
GTTCTCG1456800.038.252485
TCGCATC1479700.037.5772179
TCTCGCA1488250.037.484487
CTCGCAT1485400.037.4361848
GGTTCTC1496450.037.3647654
Produced by FastQC (version 0.11.5)