Worg
4R013-L2-P16-CCGTCC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P16-CCGTCC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P16-CCGTCC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20871524
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG18261208.749337135132059No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA17763758.510998046908313No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA12931186.195608907140658No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG11455835.48873671132017No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG2279041.0919375125649664No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1973980.945776647646813No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG1973850.9457143618261896No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC1915980.9179875892148557No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT1556110.7455660640785023No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG1396040.6688730540232711No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG1362930.6530093346322003No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT948100.454255281023082No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT921410.44146752292741054No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC904620.43342306963305605No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA901570.4319617484568927No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT886840.4249042858585698No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA855730.4099988098617044No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA788540.37780662303337315No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA755330.361894991472592No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA677480.3245953673531458No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC637640.3055071589405738No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA620630.2973572988728566No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA606280.2904819025194327No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG522420.2503027570003992No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT512580.24558819950090852No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC450470.21582995089385903No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA435410.20861437813549216No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCC434650.2082502456456941No Hit
TTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA402130.1926692080559139No Hit
CAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT387450.18563570154244607No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC371130.1778164354457298No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA359540.17226341497630934No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATT340180.16298761892040084No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT317490.15211634761314027No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG301060.1442443781297427No Hit
AACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCA287550.13777144400188504No Hit
GTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG286060.13705755267320202No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG282280.13524647265815376No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC273520.13104936659153402No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC237360.11372432602429991No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG230030.11021236398453703No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA225250.10792216227238606No Hit
GGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG219570.10520075103284264No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA214500.10277160402853189No Hit
CGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA209720.10048140231638092No Hit
CGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC209250.10025621511874265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT1334250.084.7071
CGACGCT168250.080.15021
GGATCTC1451650.077.717382
GACGCTC468200.072.675082
GATCTCT1583200.071.2363
ACGCTCA490650.069.65633
TCTCTTG1662950.067.216155
ATCTCTT1676850.066.7944644
GCTCAAA2073650.061.3011131
GCGCAGA51500.060.8638694
GCGCCCC2570600.060.50552794
CTCTTGG1861700.060.067616
CTGCATG63100.058.9843378
GCATTTC2996600.057.20150494
CTCAGAT79150.054.376641
TCTTGGT2120250.052.6911667
AGACGCT94900.052.2447741
CGTGCGT38650.051.140381
CGCTCAA680050.050.8502544
CGCCGGG10850.048.05521
Produced by FastQC (version 0.11.5)