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4R013-L2-P17-GTCCGC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P17-GTCCGC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P17-GTCCGC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19555293
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG8081904.132845260871315No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA6952613.5553596665618867No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA6770113.462034549929781No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG6006593.0715929441711767No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC1344820.6877012786256897No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG1315490.6727027817992807No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1150980.5885772204998412No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG749750.3834000339447739No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT701610.3587826579739818No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG688170.35190983842584206No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG664130.3396164915555088No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG584670.29898299145914103No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT488550.2498300587978917No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC479440.24517147352381782No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC448650.22942637576435188No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA428120.21892793935636762No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA425570.21762394457602857No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC389070.19895892124960746No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC353310.1806723120947357No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT350820.1793989995445223No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA336360.17200458208424696No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA329160.1683227144691721No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT318120.16267718412605733No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT313820.1604782909670543No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA285670.1460832113331158No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC285060.14577127532683862No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG284160.14531104187495428No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG279520.14293828274523937No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG278060.1421916818121825No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA277740.14202804325151253No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT271390.13878084056321735TruSeq Adapter, Index 18 (97% over 40bp)
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA266990.13653081035400494No Hit
TGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAGACACGGATT264690.13535465819918935No Hit
GCTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATC251690.12870684167197086No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT246210.12590453132049723No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG245000.12528577301296379No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC233540.11942546705896967No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA215360.11012875133090566No Hit
CCCAGAACTACCTTGCCGCACCAGACAGAGAGACGGATTCCTCACGCCTC208620.1066821141467939No Hit
CAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCGCC206800.10575141983298333No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA201360.10296956430159343No Hit
CCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAACAG197660.10107749344384663No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT195740.10009566207982666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT730600.080.5442051
GGATCTC800200.073.4862752
CGACGCT103500.067.5032041
GATCTCT893000.065.844193
GCGCCCC1101250.064.5947994
GACGCTC271600.061.533272
TCTCTTG964750.060.619315
ATCTCTT977250.059.9073684
CTGCATG80150.059.277538
CGCCGGG17900.059.049891
ACGCTCA296000.056.730713
GCTCAAA1211150.056.605381
CTCTTGG1079150.054.2303546
GCATTTC1221450.053.8752194
CTCAGAT98750.051.7906761
TGCATGT103500.046.1313139
TCTTGGT1273050.046.0582247
GATCTGC121050.041.813525
TCAGATC119050.041.4907532
CGCTCAA414750.041.2012064
Produced by FastQC (version 0.11.5)