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4R013-L2-P11-GGCTAC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P11-GGCTAC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P11-GGCTAC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23232559
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG10175354.3797801180662015No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA9378374.036735686327106No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA9129513.929618773377483No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG7932023.4141826563315734No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC2146380.9238672330499624No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG1885720.8116712412093734No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1433940.6172113885517303No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT1211570.5214965772819086No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG919630.3958367220761174No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG855360.36817295933693744No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG783000.33702701454454503No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA696930.2999798687695144No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG653400.2812432328268272No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT650680.28007246209941833No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC583350.25109158229190337No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA559130.2406665576529904No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC488840.21041160381858925TruSeq Adapter, Index 11 (100% over 50bp)
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA470410.20247877127956504No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT430560.18532611926219578No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA427740.18411230549333804No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA427620.1840606538435994No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC418400.18009208542201485No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG406620.1750216151393396No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC394350.1697402339535649No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT382730.1647386325372078No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT375120.16146305708294983No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC363180.15632371793395639No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC362720.15612571994329166No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA342700.1475085030452306No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT338340.1456318264380605No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA335410.14437066532360898No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA317950.13685535028663867No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG309210.13309338846400864No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC283810.12216045593599913No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC277820.11958217775321263No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG268690.11565234806893206No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA255030.10977266860701827No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG252310.10860189787960939No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCC244050.10504654265593386No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT240970.10372081697930909No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG240680.1035959921591074No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA238070.10247256877729226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT964000.080.386451
GGATCTC1048450.073.7726752
CGACGCT130950.069.713921
GATCTCT1170900.066.109743
GACGCTC350250.064.316422
GCGCCCC1379250.061.57132794
TCTCTTG1277100.060.1910175
ACGCTCA376650.060.0952533
ATCTCTT1292600.059.5827374
GCTCAAA1668600.054.7306481
CTCTTGG1452450.052.962646
GCATTTC1735750.051.36746694
CGCCGGG18700.050.1871261
CGTGCGT37250.048.8622741
CTGCATG73300.048.8527878
TCTTGGT1685150.045.692647
CGGGCGC12450.043.401451
GCGCAGA42850.042.6616994
CTCAGAT99100.041.994231
AGACGCT101350.041.8102261
Produced by FastQC (version 0.11.5)