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4R013-L2-P12-CTTGTA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P12-CTTGTA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P12-CTTGTA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7513202
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA4128585.49510049110885No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG3655824.865861452946427No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA3058004.070168750953322No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG2935283.9068296047410946No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC864461.1505879916445745No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG643070.8559200191875581No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT599170.7974895390806742No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT484840.6453174026200813No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG379520.5051374899809694No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG350290.4662326395590056No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG301330.4010673478498249No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA289780.3856944083228429No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC235720.31374106539395585No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT229880.3059680812521745No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA226860.30194849013776015No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA210560.2802533460434047No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT168390.22412547938948No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA166190.22119730043195962No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA166080.22105089148408363No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC162310.2160330575432419No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG159660.21250593288986508No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT148070.19707975374547362No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT145930.19423143421406744No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC142390.18951972807333012No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA126530.16841021977047868No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA126370.16819726130084084No Hit
GTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG123660.16459027722134983No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT117480.15636475633158806No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC115380.15356967641759134No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG114020.15175952942566964No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC107140.14260231523124228No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA105860.14089864747413952No Hit
AACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCA100910.13431024481971868No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCC94420.12567211689503358No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG93490.12443429579026359No Hit
GGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA91290.12150611683274322No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA88850.11825850017076608No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA86980.11576954805687376No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC85470.11375975249966659No Hit
TTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA84580.11257517101230607No Hit
ACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAC82690.11005959908970903No Hit
GGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG75470.10044984814730125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT364350.078.717651
GGATCTC392900.073.0406652
CGACGCT50300.071.8866961
GACGCTC134850.068.31172
GATCTCT443050.064.794223
ACGCTCA143150.064.7777253
ATCTCTT478450.059.6948474
TCTCTTG477800.059.686345
GCTCAAA697200.058.2322241
GCGCCCC533300.057.91253794
CTGCATG28600.055.20998
CTCTTGG538550.052.985636
CTCAGAT34700.049.439161
GCATTTC617550.048.10168594
GCGCAGA15150.046.21896794
TCTTGGT631600.045.3125887
CGCCGGG4400.045.2364581
AGACGCT37300.044.468371
CGTGCGT15450.041.1025051
CTCAAAC1010750.040.1986242
Produced by FastQC (version 0.11.5)