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4R013-L2-P07-CAGATC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P07-CAGATC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P07-CAGATC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28309857
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG6575892.322826992732602No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA5901312.084542496982588No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA5069001.7905424248522346No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG4420351.5614172830332558No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC927330.32756435329221195No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG914970.32319838281062313No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC866200.3059711675689496TruSeq Adapter, Index 7 (100% over 50bp)
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT812110.2868647482041326No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG721690.2549253427878495No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG680890.2405134013923136No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT544410.19230404448881533No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG525990.18579747682936018No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG489120.17277374449471788No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA471530.1665603609371817No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC411360.1453062797173437No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT370420.13084488558172513No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC355450.1255569747314513No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA351420.12413344228478441No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC346340.12233901428749712No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT319380.11281582948299597No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT294780.10412627658274642No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC294140.10390020691379684No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA289200.10215523165659225No Hit
CAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCGCC286750.10128980870514465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT565800.077.926611
GGATCTC607900.072.278982
GATCTCT703400.062.7060363
GCGCCCC903400.062.5529794
GACGCTC227550.056.7569242
ACGCTCA253300.051.2281463
TCTCTTG857500.051.189335
ATCTCTT868150.050.6269574
GCATTTC1146950.050.06908494
GCTCAAA1011200.049.104991
CTCTTGG905700.048.4631426
CGACGCT113900.047.8519741
CGCCGGG20400.047.6265831
TCTTGGT1113450.039.8801277
GTTCTCG1616250.039.8101045
TCGCATC1628400.039.403999
CTCGCAT1640350.039.1398478
TCTCGCA1652750.038.9912077
GGTTCTC1668650.038.6759154
TTCTCGC1690450.038.116756
Produced by FastQC (version 0.11.5)