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4R013-L2-P09-GATCAG-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P09-GATCAG-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P09-GATCAG-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14133304
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG3520052.4906065842778164No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA2974852.1048510666720253No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA2737631.937006378692484No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG2477631.7530437327322754No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG578620.40940179309806113No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC550480.3894913744160601No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT474140.3354771113675896No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG377750.2672764981210338No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT328420.2323731237932758No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG302220.2138353494695932No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG271060.19178813389990054No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG232150.16425741638331703No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA222260.15725976035044603No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC221010.1563753245525604No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT202630.14337058058045024No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC196080.13873613699952964No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC188720.1335285790215791No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA186180.1317314054802755No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT177100.1253068638444344No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG162570.11502618212981197No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA150720.1066417307658563No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC149850.10602616345052791TruSeq Adapter, Index 9 (100% over 50bp)
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC149180.10555210586286122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT316400.078.173671
GGATCTC344800.071.6706852
GCGCCCC470750.063.50073294
GATCTCT394350.062.831913
GACGCTC115850.056.634052
TCTCTTG467650.052.671085
ATCTCTT471800.052.407574
ACGCTCA126450.052.2208823
CGACGCT52650.051.9382321
GCTCAAA546200.050.2818681
GCATTTC582050.049.50108394
CTCTTGG506800.048.6455426
CGCCGGG11150.041.65441
TCTTGGT625500.039.9018147
GTTCTCG887200.038.7447135
TCGCATC894750.038.372649
CTCGCAT899700.038.1458478
TCTCGCA907050.037.9196437
GGTTCTC921850.037.390764
TTCTCGC921750.037.3104636
Produced by FastQC (version 0.11.5)